Abstract:

To investigate the differential expressions of vitellogenin，immunosuppressive ovarian message protein，tyrosine-protein kinase and serine/threonine-protein phosphatase genes in germinal tissues of Toxocara canis，which were designated as Tc-vit，Tc-iom，Tc-tpk and Tc-stp，the SYBR Green I real-time quantitative PCR methods were applied to analyze the different transcription of these genes in ovary，oviduct and uterus of female adult T.canis，spermary，seminal vesicle and vas deferens of male adult T.canis.The quantitative results showed that Tc-vit was transcribed in all the gremline tissues except for vas eferens；Tc-iom was transcribed in all the female germinal tissues and spermary，seminal vesicle，but not in vas deferens；the transcription of Tc-tpk in male sperm-production tissues were higher than that of female tissues；Tc-stp was highly transcribed in spermary and vas deferens and opposite in all the female germline tissues.These results laid down the foundation for the further research of gender-related genes of T.canis.